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total rabbit anti igf 1 receptor beta  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc total rabbit anti igf 1 receptor beta
    Effects of Igs purified from normal volunteers (NV-Igs) and GO patients (GO-Igs) on the levels of pERK1/2 and pAKT in U2OS-TSHR cells. U2OS-TSHR cells were incubated in HBSS (Basal) or stimulated by 100 ng/mL of insulin-like growth factor 1 <t>(IGF-1),</t> 100 mIU/mL of thyrotropin (TSH), 1:5 diluted NV-Igs (NV#), or 1:5 diluted GO-Igs (GOB# or GOM#), as described in the Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. pERK1/2 levels (gray bars) and pAKT levels (black bars) were plotted as pg/well (M ± SD) in three experiments.
    Total Rabbit Anti Igf 1 Receptor Beta, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 712 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total rabbit anti igf 1 receptor beta/product/Cell Signaling Technology Inc
    Average 96 stars, based on 712 article reviews
    total rabbit anti igf 1 receptor beta - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Evidence That Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors"

    Article Title: Evidence That Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors

    Journal: Thyroid

    doi: 10.1089/thy.2018.0089

    Effects of Igs purified from normal volunteers (NV-Igs) and GO patients (GO-Igs) on the levels of pERK1/2 and pAKT in U2OS-TSHR cells. U2OS-TSHR cells were incubated in HBSS (Basal) or stimulated by 100 ng/mL of insulin-like growth factor 1 (IGF-1), 100 mIU/mL of thyrotropin (TSH), 1:5 diluted NV-Igs (NV#), or 1:5 diluted GO-Igs (GOB# or GOM#), as described in the Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. pERK1/2 levels (gray bars) and pAKT levels (black bars) were plotted as pg/well (M ± SD) in three experiments.
    Figure Legend Snippet: Effects of Igs purified from normal volunteers (NV-Igs) and GO patients (GO-Igs) on the levels of pERK1/2 and pAKT in U2OS-TSHR cells. U2OS-TSHR cells were incubated in HBSS (Basal) or stimulated by 100 ng/mL of insulin-like growth factor 1 (IGF-1), 100 mIU/mL of thyrotropin (TSH), 1:5 diluted NV-Igs (NV#), or 1:5 diluted GO-Igs (GOB# or GOM#), as described in the Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. pERK1/2 levels (gray bars) and pAKT levels (black bars) were plotted as pg/well (M ± SD) in three experiments.

    Techniques Used: Purification, Incubation

    Effects of GO-Igs on the levels of pAKT in U2OS cells. U2OS cells were incubated in HBSS or stimulated by 100 ng/mL of IGF-1 or 1:5 diluted GO-Igs (GOB# or GOM#), as described in the Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. pAKT levels were plotted as %IGF-1 effect after subtracting basal pAKT (M ± SD) in five experiments.
    Figure Legend Snippet: Effects of GO-Igs on the levels of pAKT in U2OS cells. U2OS cells were incubated in HBSS or stimulated by 100 ng/mL of IGF-1 or 1:5 diluted GO-Igs (GOB# or GOM#), as described in the Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. pAKT levels were plotted as %IGF-1 effect after subtracting basal pAKT (M ± SD) in five experiments.

    Techniques Used: Incubation

    Effect of knockdown of IGF-1R on GO-Igs stimulation of pAKT formation in U2OS cells. siRNA was used to knockdown IGF-1R levels in U2OS cells, as described in the Materials and Methods. Control cells, transfected with non-targeting siRNA (NT), and cells in which IGF-1R was decreased by transfection with IGF-1R siRNA (IGF-1R) were incubated in HBSS or stimulated by 100 ng/mL of IGF-1, 100 mIU/mL of TSH, or 1:9 diluted GO-Igs (GOB# and GOM#), as described in Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. (A) mRNA and protein levels of IGF-1R in NT (gray bars) and IGF-1R siRNA-treated cells (black bars). WB, Western blot. (B) pAKT levels in NT cells (gray bars) and pAKT levels in IGF-1R knockdown cells (black bars) were plotted as %IGF-1 effect in NT cells (M ± SD) in three experiments.
    Figure Legend Snippet: Effect of knockdown of IGF-1R on GO-Igs stimulation of pAKT formation in U2OS cells. siRNA was used to knockdown IGF-1R levels in U2OS cells, as described in the Materials and Methods. Control cells, transfected with non-targeting siRNA (NT), and cells in which IGF-1R was decreased by transfection with IGF-1R siRNA (IGF-1R) were incubated in HBSS or stimulated by 100 ng/mL of IGF-1, 100 mIU/mL of TSH, or 1:9 diluted GO-Igs (GOB# and GOM#), as described in Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. (A) mRNA and protein levels of IGF-1R in NT (gray bars) and IGF-1R siRNA-treated cells (black bars). WB, Western blot. (B) pAKT levels in NT cells (gray bars) and pAKT levels in IGF-1R knockdown cells (black bars) were plotted as %IGF-1 effect in NT cells (M ± SD) in three experiments.

    Techniques Used: Transfection, Incubation, Western Blot



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    total rabbit anti igf 1 receptor beta  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc total rabbit anti igf 1 receptor beta
    Effects of Igs purified from normal volunteers (NV-Igs) and GO patients (GO-Igs) on the levels of pERK1/2 and pAKT in U2OS-TSHR cells. U2OS-TSHR cells were incubated in HBSS (Basal) or stimulated by 100 ng/mL of insulin-like growth factor 1 <t>(IGF-1),</t> 100 mIU/mL of thyrotropin (TSH), 1:5 diluted NV-Igs (NV#), or 1:5 diluted GO-Igs (GOB# or GOM#), as described in the Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. pERK1/2 levels (gray bars) and pAKT levels (black bars) were plotted as pg/well (M ± SD) in three experiments.
    Total Rabbit Anti Igf 1 Receptor Beta, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total rabbit anti igf 1 receptor beta/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    total rabbit anti igf 1 receptor beta - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Effects of Igs purified from normal volunteers (NV-Igs) and GO patients (GO-Igs) on the levels of pERK1/2 and pAKT in U2OS-TSHR cells. U2OS-TSHR cells were incubated in HBSS (Basal) or stimulated by 100 ng/mL of insulin-like growth factor 1 (IGF-1), 100 mIU/mL of thyrotropin (TSH), 1:5 diluted NV-Igs (NV#), or 1:5 diluted GO-Igs (GOB# or GOM#), as described in the Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. pERK1/2 levels (gray bars) and pAKT levels (black bars) were plotted as pg/well (M ± SD) in three experiments.

    Journal: Thyroid

    Article Title: Evidence That Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors

    doi: 10.1089/thy.2018.0089

    Figure Lengend Snippet: Effects of Igs purified from normal volunteers (NV-Igs) and GO patients (GO-Igs) on the levels of pERK1/2 and pAKT in U2OS-TSHR cells. U2OS-TSHR cells were incubated in HBSS (Basal) or stimulated by 100 ng/mL of insulin-like growth factor 1 (IGF-1), 100 mIU/mL of thyrotropin (TSH), 1:5 diluted NV-Igs (NV#), or 1:5 diluted GO-Igs (GOB# or GOM#), as described in the Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. pERK1/2 levels (gray bars) and pAKT levels (black bars) were plotted as pg/well (M ± SD) in three experiments.

    Article Snippet: Total rabbit anti-IGF-1 receptor beta (#3027S) was purchased from Cell Signaling Technologies (Danvers, MA).

    Techniques: Purification, Incubation

    Effects of GO-Igs on the levels of pAKT in U2OS cells. U2OS cells were incubated in HBSS or stimulated by 100 ng/mL of IGF-1 or 1:5 diluted GO-Igs (GOB# or GOM#), as described in the Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. pAKT levels were plotted as %IGF-1 effect after subtracting basal pAKT (M ± SD) in five experiments.

    Journal: Thyroid

    Article Title: Evidence That Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors

    doi: 10.1089/thy.2018.0089

    Figure Lengend Snippet: Effects of GO-Igs on the levels of pAKT in U2OS cells. U2OS cells were incubated in HBSS or stimulated by 100 ng/mL of IGF-1 or 1:5 diluted GO-Igs (GOB# or GOM#), as described in the Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. pAKT levels were plotted as %IGF-1 effect after subtracting basal pAKT (M ± SD) in five experiments.

    Article Snippet: Total rabbit anti-IGF-1 receptor beta (#3027S) was purchased from Cell Signaling Technologies (Danvers, MA).

    Techniques: Incubation

    Effect of knockdown of IGF-1R on GO-Igs stimulation of pAKT formation in U2OS cells. siRNA was used to knockdown IGF-1R levels in U2OS cells, as described in the Materials and Methods. Control cells, transfected with non-targeting siRNA (NT), and cells in which IGF-1R was decreased by transfection with IGF-1R siRNA (IGF-1R) were incubated in HBSS or stimulated by 100 ng/mL of IGF-1, 100 mIU/mL of TSH, or 1:9 diluted GO-Igs (GOB# and GOM#), as described in Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. (A) mRNA and protein levels of IGF-1R in NT (gray bars) and IGF-1R siRNA-treated cells (black bars). WB, Western blot. (B) pAKT levels in NT cells (gray bars) and pAKT levels in IGF-1R knockdown cells (black bars) were plotted as %IGF-1 effect in NT cells (M ± SD) in three experiments.

    Journal: Thyroid

    Article Title: Evidence That Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors

    doi: 10.1089/thy.2018.0089

    Figure Lengend Snippet: Effect of knockdown of IGF-1R on GO-Igs stimulation of pAKT formation in U2OS cells. siRNA was used to knockdown IGF-1R levels in U2OS cells, as described in the Materials and Methods. Control cells, transfected with non-targeting siRNA (NT), and cells in which IGF-1R was decreased by transfection with IGF-1R siRNA (IGF-1R) were incubated in HBSS or stimulated by 100 ng/mL of IGF-1, 100 mIU/mL of TSH, or 1:9 diluted GO-Igs (GOB# and GOM#), as described in Materials and Methods. GOB and GOM stand for patient samples from Bethesda, MD, and Mainz, Germany, respectively. (A) mRNA and protein levels of IGF-1R in NT (gray bars) and IGF-1R siRNA-treated cells (black bars). WB, Western blot. (B) pAKT levels in NT cells (gray bars) and pAKT levels in IGF-1R knockdown cells (black bars) were plotted as %IGF-1 effect in NT cells (M ± SD) in three experiments.

    Article Snippet: Total rabbit anti-IGF-1 receptor beta (#3027S) was purchased from Cell Signaling Technologies (Danvers, MA).

    Techniques: Transfection, Incubation, Western Blot